protocols:cryo
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diluting_pooled_sperm_based_on_nanodrop_cell_counts_04.24.2023.pdf

Cryopreservation and In Vitro Fertilization (IVF) Protocol

  • ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol PDF
    • Complete description of ZIRC's current cryopreservation & IVF protocols
  • ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator XLS
    • ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender
  • Diluting Pooled Sperm based on Nano Drop Cell Counts PDF
    • Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet
  • Pooled Sperm Dilution Worksheet XLS
    • A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts 04.24.2003.pdf
protocols/cryo.1682626676.txt.gz · Last modified: 2023/04/27 13:17 by ron

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