protocols:cryo

Cryopreservation and In Vitro Fertilization (IVF) Protocols

Current Protocols

  • ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 PDF
    • A step-by-step description of ZIRC's current cryopreservation & IVF protocols
  • Calibration Curve and Sperm Density Calculator XLS
    • Sperm Density Calculator for use with Nanodrop Spectrophotometer concentration determination

Previous Protocols

  • Cryopreservation of zebra fish spermatozoa using methanol by Harvey et al. 1982 PDF
    • The Canadian Journal of Zoology has kindly permitted us to provide a downloadable document of the original article by Harvey et al., (1982) that describes cryopreservation for zebrafish. All copyrights remain with the Canadian Journal of Zoology. You can download the article for your own use. Please inform yourself here about copyright issues.
  • Sperm Cryopreservation by Walker and Streisinger
  • Sperm Cryopreservation by Draper and Moens. 2004
    • View Draper and Moens' complete article and protocol video (A high-throughput method for zebrafish sperm cryopreservation and in vitro fertilization) on the Fred Hutchinson Cancer Research Center web site. This is the protocol on which we base our procedures here at ZIRC (see 4, below).
  • Cryopreservation and In Vitro Fertilization at the ZIRC by Carmichael et al. 2009 PDF
    • This review provides a step-by-step description of the Draper and Moens method (see 3, above) as it is currently applied at the ZIRC. In addition, we include detailed notes with background, advice, and additional information. The authors, and ZIRC in general, wish to express their gratitude to Cecilia and Bruce for teaching us the protocol and for their continued advice and support.
protocols/cryo.txt · Last modified: 2019/11/01 10:52 by rubin