Cryopreservation and In Vitro Fertilization (IVF) Protocols [ZIRC Public Wiki]

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====== Cryopreservation and In Vitro Fertilization (IVF) Protocols ======

  *  {{ :protocols:zirc_e400_rmmb_sperm_cryopreservation_ivf_protocol_04.24.2023.pdf |ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol}}
      * Complete description of ZIRC's current cryopreservation & IVF protocols

  * [[:protocols:cryo:step-by-step_cryopreservation_protocol]]
      * Abridged version of ZIRC's cryopreservation & IVF protocols

  *  {{ :protocols:zirc_nanodrop_2000_calibration_curve_and_sperm_density_calculator.xlsx | ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator}}
      * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender

  *  {{ :protocols:diluting_pooled_sperm_based_on_nanodrop_cell_counts_04.24.2023.pdf |Diluting Pooled Sperm based on Nano Drop Cell Counts}}
      * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet

  * {{ :protocols:pooled_sperm_dilution_worksheet_blank.xlsx |Pooled Sperm Dilution Worksheet}}
      * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts

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