protocols:cryo
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Cryopreservation and In Vitro Fertilization (IVF) Protocol

  • ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol 04.24.2023.pdf
    • Complete description of ZIRC's current cryopreservation & IVF protocols
  • Step-by-Step Cryopreservation: Pooling, Freezing, Thawing, and IVF Protocols
    • Abridged version of ZIRC's cryopreservation & IVF protocols
  • ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator
    • ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender
  • Diluting Pooled Sperm based on Nano Drop Cell Counts 04.24.2023.pdf
    • Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet
  • Pooled Sperm Dilution Worksheet Worksheet.xlsx
    • A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Duluting Pooled Sperm based on NanoDrop Cell Counts 04.24.2003.pdf
  • ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 PDF
    • A step-by-step description of ZIRC's current cryopreservation & IVF protocols
  • ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator XLS
    • Sperm concentration calculator for use with NanoDrop 2000 Spectrophotometer OD400 measurements for stripped sperm in E400 extender
protocols/cryo.1682621095.txt.gz · Last modified: 2023/04/27 11:44 by ron

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