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Cryopreservation and In Vitro Fertilization (IVF) Protocols
ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 PDF
Calibration Curve and Sperm Density Calculator XLS
ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator XLS
Cryopreservation of zebra fish spermatozoa using methanol by Harvey et al. 1982 PDF
The Canadian Journal of Zoology
has kindly permitted us to provide a downloadable document of the original article by Harvey et al., (1982) that describes cryopreservation for zebrafish. All copyrights remain with the Canadian Journal of Zoology. You can download the article for your own use. Please inform yourself here
about copyright issues.
Cryopreservation and In Vitro Fertilization at the ZIRC by Carmichael et al. 2009 PDF
This review provides a step-by-step description of the Draper and Moens method (see 3, above) as it is currently applied at the ZIRC. In addition, we include detailed notes with background, advice, and additional information. The authors, and ZIRC in general, wish to express their gratitude to Cecilia and Bruce for teaching us the protocol and for their continued advice and support.