This shows you the differences between two versions of the page.
Both sides previous revisionPrevious revisionNext revision | Previous revisionNext revision | ||
protocols:cryo [2020/01/16 10:09] ron [Current Protocols] | protocols:cryo [2021/10/29 19:41] zoltan | ||
---|---|---|---|
Line 1: | Line 1: | ||
- | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols | + | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocol |
- | ===== Current Protocols ===== | ||
* ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 {{: | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 {{: | ||
* A step-by-step description of ZIRC's current cryopreservation & IVF protocols | * A step-by-step description of ZIRC's current cryopreservation & IVF protocols | ||
- | | + | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator {{: |
- | * Sperm Density Calculator for use with Nanodrop Spectrophotometer concentration determination | + | |
- | + | ||
- | | + | |
* Sperm concentration calculator for use with NanoDrop 2000 Spectrophotometer OD400 measurements for stripped sperm in E400 extender | * Sperm concentration calculator for use with NanoDrop 2000 Spectrophotometer OD400 measurements for stripped sperm in E400 extender | ||
- | ===== Previous Protocols ===== | + | |
- | * Cryopreservation of zebra fish spermatozoa using methanol by Harvey et al. 1982 {{: | + | |
- | * [[http:// | + | |
- | + | ||
- | * Sperm Cryopreservation by Walker and Streisinger | + | |
- | * The laboratory protocol that was adapted from Harvey et al. by Walker and Streisinger can be found in [[http:// | + | |
- | + | ||
- | * Sperm Cryopreservation by Draper and Moens. 2004 | + | |
- | * View Draper and Moens' [[http:// | + | |
- | + | ||
- | * Cryopreservation and In Vitro Fertilization at the ZIRC by Carmichael et al. 2009 {{: | + | |
- | * This review provides a step-by-step description of the Draper and Moens method (see 3, above) as it is currently applied at the ZIRC. In addition, we include detailed notes with background, advice, and additional information. The authors, and ZIRC in general, wish to express their gratitude to Cecilia and Bruce for teaching us the protocol and for their continued advice and support. | + |