| Antibody Labeling |
| HIAR |
 pdf |
| Heat Induced Antigen Retrieval (HIAR)
This protocol describes a pretreatment of tissues for optimal antigen target preservation that is used for some monoclonal antibodies. |
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| Wholemount Antibody & ABC reaction |
pdf |
| This is a standard labeling protocol for antibody labeling and additional ABC staining. |
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| Wholemount Antibody Labeling |
pdf |
| This is a standard labeling protocol derived from the Zebrafish Book (Westerfield, 2000). |
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| Approved IACUC Animal Use Protocols |
| ZIRC |
pdf |
| This is the approved animal use protocol for the Zebrafish International Resource Center in Eugene, Oregon, USA. |
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| Amacher Lab |
pdf |
| This is the approved animal use protocol for the Amacher lab at the University of California at Berkeley, located in Berkeley, California, USA. |
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| Burgess Lab |
pdf |
| This is the approved animal use protocol for the Burgess lab at the National Institutes of Health, located in Bethesda, Maryland, USA. |
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| Cryopreservation and In Vitro Fertilization (IVF) |
| 1. Cryopreservation of zebra fish spermatozoa using methanol by Harvey et al. 1982 |
pdf |
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The Canadian Journal of Zoology
has kindly permitted us to provide a downloadable document of the original
article by Harvey et al., (1982) that describes cryopreservation for
zebrafish. All copyrights remain with the Canadian Journal of Zoology. You
can download the article for your own use. Please
inform yourself here
about copyright issues. |
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| 2. Sperm Cryopreservation by Walker and Streisinger |
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| The laboratory protocol that was adapted
from Harvey et al. by Walker and Streisinger can be found
in
the Zebrafish Book, Chapter 7. |
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| 3. Sperm Cryopreservation by Draper and Moens. 2004 |
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| View Draper and Moens' complete article and protocol video
(A high-throughput method for zebrafish sperm cryopreservation and in vitro fertilization)
on the Fred Hutchinson Cancer Research Center web site. This is the protocol on which we base our procedures
here at ZIRC (see 4, below). |
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| 4. Cryopreservation and In Vitro Fertilization at the ZIRC by Carmichael et al. 2009 |
pdf |
| This review provides a step-by-step description of the Draper and Moens method
(see 3, above) as it is currently applied at the ZIRC. In addition, we include detailed notes with background, advice,
and additional information. The authors, and ZIRC in general, wish to express their gratitude to Cecilia and Bruce
for teaching us the protocol and for their continued advice and support. |
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| Egg Bleaching |
| Egg Bleaching |
pdf |
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| Fish Feeding |
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Dry Food |
| Dry Food Recipes |
pdf |
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Live Food |
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Artemia |
| Artemia Decapsulation |
pdf |
| Decapsulated Artemia Hatching |
pdf |
| Non-Decapsulated Artemia Hatching |
pdf |
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Paramecia |
| ZIRC Paramecia Instructions |
pdf |
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Schedules and Suppliers |
| Feeding Schedule - weekday and weekend |
pdf |
| Food Source Information |
pdf |
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| Fish Nursery |
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| E2 Solution |
pdf |
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Nursery |
| Basic Nursery Instructions |
pdf |
| Detailed Nursery and Food Instructions |
pdf |
| Nursery Protocol, Siphon Method |
pdf |
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| Genotyping |
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| A. Read this first! |
pdf |
| B. Overview of Genotyping Assays at ZIRC |
pdf |
| C. Designing and Handling of PCR Primers |
pdf |
| D. PCR Sample Preparation |
pdf |
| E. General PCR Protocol |
pdf |
| F. Restriction Enzyme Digestion |
pdf |
| G. Gel Electrophoresis |
pdf |
| H. Controls |
pdf |
| Files A. - H. Combined |
pdf |
I. Find and Download Line Specific
Genotyping Protocols here |
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We provide all protocols we use at ZIRC online as soon
as possible. If you see a fish line that has no protocol
associated with it on our pages, it means that it probably
does not exist. Please be reminded that we are unable to
develop and provide specific PCR protocols upon request. |
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| ZIRC Health Monitoring SOPs |
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| Sentinel Fish Program |
pdf |
| Daily Monitoring of Fish Morbidity and Mortality SOP |
pdf |
| Fixing Zebrafish for Histopathology |
pdf |
| ZIRC PCR protocol for P. neurophilia |
pdf |
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| Labels |
| ZIRC Label Info |
pdf |
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