This shows you the differences between two versions of the page.
Both sides previous revisionPrevious revisionNext revision | Previous revisionBoth sides next revision | ||
protocols:cryo [2023/04/27 13:16] ron [Cryopreservation and In Vitro Fertilization (IVF) Protocol] | protocols:cryo [2023/04/27 13:41] (current) ron | ||
---|---|---|---|
Line 1: | Line 1: | ||
- | {{ : | + | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols |
- | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocol | + | |
- | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol | + | * {{ : |
* Complete description of ZIRC's current cryopreservation & IVF protocols | * Complete description of ZIRC's current cryopreservation & IVF protocols | ||
- | * Step-by-Step Cryopreservation: | + | * [[: |
* Abridged version of ZIRC's cryopreservation & IVF protocols | * Abridged version of ZIRC's cryopreservation & IVF protocols | ||
- | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator | + | * {{ : |
* ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | ||
- | * Diluting Pooled Sperm based on Nano Drop Cell Counts | + | * {{ : |
* Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | ||
- | * Pooled Sperm Dilution Worksheet | + | * {{ : |
- | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts | + | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts |
- | ---------------- | ||
- | |||
- | * Step-by-Step Cryopreservation: | ||
- | * [[: | ||
| |