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protocols:cryo [2023/04/27 11:44] ron | protocols:cryo [2023/04/27 13:41] (current) ron | ||
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- | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocol | + | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols |
- | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol | + | * {{ : |
* Complete description of ZIRC's current cryopreservation & IVF protocols | * Complete description of ZIRC's current cryopreservation & IVF protocols | ||
- | * Step-by-Step Cryopreservation: | + | * [[: |
* Abridged version of ZIRC's cryopreservation & IVF protocols | * Abridged version of ZIRC's cryopreservation & IVF protocols | ||
- | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density | + | * {{ : |
* ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | ||
- | * Diluting Pooled Sperm based on Nano Drop Cell Counts | + | * {{ : |
* Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | ||
- | * Pooled Sperm Dilution Worksheet | + | * {{ : |
- | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Duluting | + | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting |
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- | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 {{: | ||
- | * A step-by-step description of ZIRC's current cryopreservation & IVF protocols | ||
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- | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator {{: | ||
- | * Sperm concentration calculator for use with NanoDrop 2000 Spectrophotometer OD400 measurements for stripped sperm in E400 extender | ||
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- | * Step-by-Step Cryopreservation: | ||
- | * [[step-by-step_cryopreservation_protocol]] | ||
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