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protocols:cryo [2021/10/29 19:41] zoltan | protocols:cryo [2023/04/27 13:41] ron | ||
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- | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocol | + | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols |
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+ | * Complete description of ZIRC's current cryopreservation & IVF protocols | ||
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+ | * Abridged version of ZIRC's cryopreservation & IVF protocols | ||
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+ | * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | ||
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+ | * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | ||
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+ | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts | ||
- | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 {{: | ||
- | * A step-by-step description of ZIRC's current cryopreservation & IVF protocols | ||
- | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator {{: | ||
- | * Sperm concentration calculator for use with NanoDrop 2000 Spectrophotometer OD400 measurements for stripped sperm in E400 extender | ||
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