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| protocols:cryo [2021/10/29 19:41] zoltan | protocols:cryo [2023/04/27 13:38] ron | ||
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| - | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocol | + | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols |
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| + | * Complete description of ZIRC's current cryopreservation & IVF protocols | ||
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| + | * Abridged version of ZIRC's cryopreservation & IVF protocols | ||
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| + | * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender | ||
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| + | * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet | ||
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| + | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts 04.24.2003.pdf | ||
| - | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 {{: | ||
| - | * A step-by-step description of ZIRC's current cryopreservation & IVF protocols | ||
| - | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator {{: | ||
| - | * Sperm concentration calculator for use with NanoDrop 2000 Spectrophotometer OD400 measurements for stripped sperm in E400 extender | ||
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