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--- protocols:cryo [2021/10/26 12:30]
zoltan
+++ protocols:cryo [2023/04/27 13:38]
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@@ Line 1: / Line 1: @@
-====== Cryopreservation and In Vitro Fertilization (IVF) Protocol ======
+====== Cryopreservation and In Vitro Fertilization (IVF) Protocols ======
-  * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18 {{:protocols:cryo:zirc_rmmb_freezing_protocol.pdf|PDF}}
+  *  {{ :protocols:zirc_e400_rmmb_sperm_cryopreservation_ivf_protocol_04.24.2023.pdf |ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol 04.24.2023}}
-    * A step-by-step description of ZIRC's current cryopreservation & IVF protocols
+      * Complete description of ZIRC's current cryopreservation & IVF protocols
-  * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator {{:protocols:cryo:nanodrop_sperm_density_calc_3rd_order_poly_curve.xlsx|XLS}}
+  * [[:protocols:cryo:step-by-step_cryopreservation_protocol]]
-    * Sperm concentration calculator for use with NanoDrop 2000 Spectrophotometer OD400 measurements for stripped sperm in E400 extender
+      * Abridged version of ZIRC's cryopreservation & IVF protocols
+  *  {{ :protocols:zirc_nanodrop_2000_calibration_curve_and_sperm_density_calculator.xlsx | ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator}}
+      * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender
+  *  {{ :protocols:diluting_pooled_sperm_based_on_nanodrop_cell_counts_04.24.2023.pdf |Diluting Pooled Sperm based on Nano Drop Cell Counts}}
+      * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet
+  * {{ :protocols:pooled_sperm_dilution_worksheet_blank.xlsx |Pooled Sperm Dilution Worksheet}}
+      * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts 04.24.2003.pdf

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