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protocols:cryo [2020/01/16 10:04] ron [Current Protocols] | protocols:cryo [2023/04/27 13:41] ron | ||
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====== Cryopreservation and In Vitro Fertilization (IVF) Protocols ====== | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols ====== | ||
- | ===== Current Protocols ===== | + | * {{ : |
- | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol | + | * Complete |
- | * A step-by-step | + | |
- | * Calibration Curve and Sperm Density Calculator {{: | + | * [[: |
- | * Sperm Density Calculator for use with Nanodrop Spectrophotometer concentration determination | + | * Abridged version of ZIRC's cryopreservation & IVF protocols |
- | * ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator | + | * {{ : |
- | * Sperm concentration calculator for use with NanoDrop 2000 | + | * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender |
- | ===== Previous Protocols ===== | + | * {{ :protocols:diluting_pooled_sperm_based_on_nanodrop_cell_counts_04.24.2023.pdf |Diluting Pooled Sperm based on Nano Drop Cell Counts}} |
- | * Cryopreservation of zebra fish spermatozoa using methanol by Harvey et al. 1982 {{: | + | * Detailed description |
- | * [[http:// | + | |
- | * Sperm Cryopreservation by Walker and Streisinger | + | * {{ : |
- | * The laboratory protocol that was adapted from Harvey et al. by Walker and Streisinger can be found in [[http:// | + | * A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described |
- | * Sperm Cryopreservation by Draper and Moens. 2004 | ||
- | * View Draper and Moens' [[http:// | ||
- | * Cryopreservation and In Vitro Fertilization at the ZIRC by Carmichael et al. 2009 {{: | + | |
- | * This review provides a step-by-step description of the Draper and Moens method (see 3, above) as it is currently applied at the ZIRC. In addition, we include detailed notes with background, advice, and additional information. The authors, and ZIRC in general, wish to express their gratitude to Cecilia and Bruce for teaching us the protocol and for their continued advice and support. | + |