protocols:shipping
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protocols:shipping [2021/12/30 11:15]
ron [3. General Packaging and Labeling] 		protocols:shipping [2021/12/30 14:26] (current)
ron [Shipping Materials]
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 When receiving a shipment of zebrafish from abroad, a custom declaration (Form 3-177) must be filed with the US Fish and Wildlife Service (USFWS). Visit the http://www.fws.gov/le/declaration-form-3-177.html site, download the four pages, fill in the form and file it with the appropriate office (see instructions). Alternatively, you may with eDecs, an electronic declaration system offered by USFWS. In this case, create a new personal account on the eDecs site https://edecs.fws.gov/eDecsHome.cfm, create an Importer/Exporter Account and a new eDec file, and file it electronically.  For academic institutions filing must occur, but a license is not needed and the fees are waived.   When receiving a shipment of zebrafish from abroad, a custom declaration (Form 3-177) must be filed with the US Fish and Wildlife Service (USFWS). Visit the http://www.fws.gov/le/declaration-form-3-177.html site, download the four pages, fill in the form and file it with the appropriate office (see instructions). Alternatively, you may with eDecs, an electronic declaration system offered by USFWS. In this case, create a new personal account on the eDecs site https://edecs.fws.gov/eDecsHome.cfm, create an Importer/Exporter Account and a new eDec file, and file it electronically.  For academic institutions filing must occur, but a license is not needed and the fees are waived.  
  
 +===== Shipping Materials =====
 +
 +
 +=== 1. Styrofoam Boxes, nested in cardboard box: ===
 +
 +  *  VWR International, https://us.vwr.com/
 +  * Small box dimensions 8" L X 6" W X 9" H; Catalog number 14100-412
 +  * Medium box dimensions 12" L X 10" W X 7" H; Catalog number 14100-402
 +  * Extra-large box dimensions 22” L X 15” W X 15” H; Catalog number 15714-704, Superior Shipping, http://superiorshippingsupplies.com/category  And http://www.thermosafe.com/.
 +
 +=== 2. Solutions: ===
 +  * Ammonia binder: ClorAM-X, Reed Mariculture, http://reedmariculture.com/product_cloram_x.php 
 +  * Sterile Fish System Water
 +
 +=== 3. Styrofoam packing peanuts: === 
 +  * do not use water dissolvable cellulose!
 +
 +=== 4. Heat Packs: ===
 +  * Superior Shipping, http://superiorshippingsupplies.com/category
 +  * For the UK: http://www.heatpacksuk.co.uk/heat-packs.php
 +  * 40 hour pack for domestic (overnight) shipments
 +  * 60-72 hour packs for international (2-4 day) shipments
 + 
 +=== 5. Live fish labels: === 
 +  * Customs and veterinary health certification documents for international shipments (Fig. B)
 +  * address, courier labels and document pouch (Fig. I)
 +
 +=== 6. Embryo Packaging: ===
 +  * 250 ml Tissue culture flasks, Fisher Scientific, catalog # 10-126-5; http://www.fishersci.com 
 +  * VWR International, catalog # 82050-854; https://us.vwr.com/ 
 +  * 0.5X Embryo Medium (E2)
 +  * Methylene Blue (optional)
 +
 +=== 7. Adult Packaging: ===
 +  * Polyethylene plastic bags: at least 0.003 mm or 1.2x10-4 inches thick). 
 +  * Gulf Coast Plastics for bulk quantity, http://www.gulfcoastplastics.com/BottomSeal.htm
 +  * Superior Shipping, http://superiorshippingsupplies.com/category
 +  * Check any pet store for small quantities
 +  * Average size for holding 10-20 fish (10" X 18" (.003 mil))
 +  * Note: Square bottom bags can be mad from the above using a plastic heat/bag sealer.  You can also tape the corners of the bag to create square bottom.
 +  * Pure oxygen (not required, recommended)
 +  * Rubber-bands, plastic ties. or metal clips
 +
 +{{ :protocols:packaging_wiki_fig.jpg?600 |}}
 +=== Figure Legend ===
 +Packaging of embryos and adults for shipping. A) Thick-walled Styrofoam box inside cardboard box, labeled as indicated in panel B).  C) Adults are prepared in labeled breeding cages, embryos in labeled 250 ml flasks. D) Adults and their label are transferred to two leak-proof polyethylene bags and closed with plenty of air or oxygen. The Styrofoam box is partially filled with Styrofoam packing peanuts before the bag or flask is added (E,F). Completely filled Styrofoam box. Packing peanuts prevent movement of bag or flask. For the cold season or for cold-climate destinations, a hole is punched into the Styrofoam lid with a pencil or screwdriver to provide oxygen for a heat pack (H) which is taped to the lid over the hole. I) Address labels and pouch with shipping documents (Invoice, Customs statement, Copy of import permit, Health Declaration/Certification) are added in triplicate copy to the pouch.
 +
 +=== Sources: ===
 +
 +//Varga, Z.M. (2016) Aquaculture, husbandry, and shipping at the Zebrafish International Resource Center. Methods in Cell Biology. 135:509-34.// \\
 +http://zfin.org/ZDB-PUB-160725-11
 +
 +and\\
 +=== The Zebrafish Book: ===
 +4th Edition (2000) Chapter 1.7 (online): 
 +http://zfin.org/zf_info/zfbook/chapt1/1.7.html
 +
 +5th Edition (2007) Pages 1.24 - 1.39 (print): 
 +http://zebrafish.org/orders/buyBookQ.php?item=Book&id=book&detail=The%20Zebrafish%20Book
protocols/shipping.1640891722.txt.gz · Last modified: 2021/12/30 11:15 by ron

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