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Cryopreservation and In Vitro Fertilization (IVF) Protocols
Current Protocols
ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol by Matthews et al. V.1.2_2017_07_18
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Calibration Curve and Sperm Density Calculator
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ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator
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Previous Protocols
Cryopreservation of zebra fish spermatozoa using methanol by Harvey et al. 1982
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The Canadian Journal of Zoology has kindly permitted us to provide a downloadable document of the original article by Harvey et al., (1982) that describes cryopreservation for zebrafish. All copyrights remain with the Canadian Journal of Zoology. You can download the article for your own use. Please
inform yourself here about copyright issues.
Cryopreservation and In Vitro Fertilization at the ZIRC by Carmichael et al. 2009
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This review provides a step-by-step description of the Draper and Moens method (see 3, above) as it is currently applied at the ZIRC. In addition, we include detailed notes with background, advice, and additional information. The authors, and ZIRC in general, wish to express their gratitude to Cecilia and Bruce for teaching us the protocol and for their continued advice and support.