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protocols:cryo [2019/11/01 10:52] rubin created | protocols:cryo [2023/04/27 13:41] (current) ron | ||
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====== Cryopreservation and In Vitro Fertilization (IVF) Protocols ====== | ====== Cryopreservation and In Vitro Fertilization (IVF) Protocols ====== | ||
- | ===== Current Protocols ===== | + | * {{ : |
- | * ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol | + | * Complete |
- | * A step-by-step | + | |
- | * Calibration Curve and Sperm Density Calculator {{: | + | * [[: |
- | * Sperm Density Calculator for use with Nanodrop Spectrophotometer concentration determination | + | * Abridged version of ZIRC's cryopreservation & IVF protocols |
- | ===== Previous Protocols ===== | + | * {{ :protocols:zirc_nanodrop_2000_calibration_curve_and_sperm_density_calculator.xlsx | ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator}} |
- | * Cryopreservation of zebra fish spermatozoa using methanol by Harvey et al. 1982 {{: | + | * ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator |
- | * [[http:// | + | |
- | * Sperm Cryopreservation by Walker and Streisinger | + | * {{ :protocols:diluting_pooled_sperm_based_on_nanodrop_cell_counts_04.24.2023.pdf |Diluting Pooled Sperm based on Nano Drop Cell Counts}} |
- | * The laboratory protocol that was adapted from Harvey et al. by Walker and Streisinger can be found in [[http://zfin.org/ | + | * Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet |
- | * Sperm Cryopreservation by Draper and Moens. 2004 | + | * {{ : |
- | * View Draper and Moens' [[http:// | + | * A blank Excel sperm dilution worksheet |
- | * Cryopreservation and In Vitro Fertilization at the ZIRC by Carmichael et al. 2009 {{: | + | |
- | * This review provides a step-by-step description of the Draper and Moens method (see 3, above) as it is currently applied at the ZIRC. In addition, we include detailed notes with background, advice, and additional information. The authors, and ZIRC in general, wish to express their gratitude to Cecilia and Bruce for teaching us the protocol and for their continued advice and support. | + | |
+ | |