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health:submission:preperation [2020/01/08 10:19] gordon [Histopathology Sample Preparation] | health:submission:preperation [2020/01/08 10:20] (current) gordon [Fixation Protocol for Histopathology Specimens] | ||
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* Euthanize fish. | * Euthanize fish. | ||
* Carefully slit open the body cavity along the belly, trying not to disrupt internal organs. | * Carefully slit open the body cavity along the belly, trying not to disrupt internal organs. | ||
- | * The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish | + | * The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish. |
* Use 10-15 ml of fixative per 1-2 fish in a sealable container. The volume of fixative should be at least 10 times the volume of the specimen. | * Use 10-15 ml of fixative per 1-2 fish in a sealable container. The volume of fixative should be at least 10 times the volume of the specimen. | ||
* Fish should be placed in a horizontal position with gentle agitation (e.g. laboratory rocker) for the first 24 hours of fixation. This will ensure optimal fixation and prevent artificial bending of the body axis. | * Fish should be placed in a horizontal position with gentle agitation (e.g. laboratory rocker) for the first 24 hours of fixation. This will ensure optimal fixation and prevent artificial bending of the body axis. |