health:submission:preperation

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health:submission:preperation [2020/01/08 10:19]
gordon [Histopathology Sample Preparation]
health:submission:preperation [2020/01/08 10:20]
gordon [Fixation Protocol for Histopathology Specimens]
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   * Euthanize fish.   * Euthanize fish.
   * Carefully slit open the body cavity along the belly, trying not to disrupt internal organs.   * Carefully slit open the body cavity along the belly, trying not to disrupt internal organs.
-  * The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish+  * The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish.
   * Use 10-15 ml of fixative per 1-2 fish in a sealable container. The volume of fixative should be at least 10 times the volume of the specimen.   * Use 10-15 ml of fixative per 1-2 fish in a sealable container. The volume of fixative should be at least 10 times the volume of the specimen.
   * Fish should be placed in a horizontal position with gentle agitation (e.g. laboratory rocker) for the first 24 hours of fixation. This will ensure optimal fixation and prevent artificial bending of the body axis.   * Fish should be placed in a horizontal position with gentle agitation (e.g. laboratory rocker) for the first 24 hours of fixation. This will ensure optimal fixation and prevent artificial bending of the body axis.
health/submission/preperation.txt · Last modified: 2020/01/08 10:20 by gordon