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health:submission:preperation [2020/01/08 10:11] gordon [Fixation Protocol for Histopathology Specimens] | health:submission:preperation [2020/01/08 10:20] gordon [Fixation Protocol for Histopathology Specimens] | ||
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Fish intended for bacteriology or necropsy exam should be shipped alive (see [[shipping|Shipping Instructions]]). Shipping containers for shipping live fish are available upon request. Bacteriology samples can also be taken from frozen specimens if necessary. We require notification prior to shipment of all live specimens to ensure appropriate personnel and lab supplies are available. | Fish intended for bacteriology or necropsy exam should be shipped alive (see [[shipping|Shipping Instructions]]). Shipping containers for shipping live fish are available upon request. Bacteriology samples can also be taken from frozen specimens if necessary. We require notification prior to shipment of all live specimens to ensure appropriate personnel and lab supplies are available. | ||
===== Histopathology Sample Preparation ===== | ===== Histopathology Sample Preparation ===== | ||
- | For optimum results, fish must be alive and euthanatized | + | For optimum results, fish must be alive and euthanized |
- | | + | |
===== Fixation Protocol for Histopathology Specimens ===== | ===== Fixation Protocol for Histopathology Specimens ===== | ||
* Prepare Dietrich' | * Prepare Dietrich' | ||
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* 2 ml Glacial Acetic Acid | * 2 ml Glacial Acetic Acid | ||
* 58 ml Distilled Water | * 58 ml Distilled Water | ||
- | * Euthanatize | + | * Euthanize |
* Carefully slit open the body cavity along the belly, trying not to disrupt internal organs. | * Carefully slit open the body cavity along the belly, trying not to disrupt internal organs. | ||
- | * The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish | + | * The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish. |
* Use 10-15 ml of fixative per 1-2 fish in a sealable container. The volume of fixative should be at least 10 times the volume of the specimen. | * Use 10-15 ml of fixative per 1-2 fish in a sealable container. The volume of fixative should be at least 10 times the volume of the specimen. | ||
* Fish should be placed in a horizontal position with gentle agitation (e.g. laboratory rocker) for the first 24 hours of fixation. This will ensure optimal fixation and prevent artificial bending of the body axis. | * Fish should be placed in a horizontal position with gentle agitation (e.g. laboratory rocker) for the first 24 hours of fixation. This will ensure optimal fixation and prevent artificial bending of the body axis. |