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health:submission:preperation [2016/04/11 16:05] rubin created | health:submission:preperation [2020/01/08 10:11] gordon [Fixation Protocol for Histopathology Specimens] | ||
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* 2 ml Glacial Acetic Acid | * 2 ml Glacial Acetic Acid | ||
* 58 ml Distilled Water | * 58 ml Distilled Water | ||
- | * Euthanatize fish with an overdose of MS-222 (tricaine methane sulfonate). | + | * Euthanatize fish. |
* Carefully slit open the body cavity along the belly, trying not to disrupt internal organs. | * Carefully slit open the body cavity along the belly, trying not to disrupt internal organs. | ||
* The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish | * The tail may be removed by cutting through the caudal peduncle well behind the anal fin. The tail should be left intact on fish with tail lesions and small fish |