Cryopreservation and In Vitro Fertilization (IVF) Protocols

ZIRC E400/RMMB Sperm Cryopreservation & IVF Protocol
- Complete description of ZIRC's current cryopreservation & IVF protocols

Step-by-Step Cryopreservation: Pooling, Freezing, Thawing, and IVF Protocols
- Abridged version of ZIRC's cryopreservation & IVF protocols

ZIRC NanoDrop 2000 Calibration Curve and Sperm Density Calculator
- ZIRC's NanoDrop 2000 hemocytometer-generated standard curve and sperm concentration calculator for use with OD400 measurements for stripped sperm in E400 extender

Diluting Pooled Sperm based on Nano Drop Cell Counts
- Detailed description of how to use a NanoDrop 2000 spectrophotometer for measuring sperm cell concentrations and how to use the Excel Pooled Sperm Dilution Worksheet

Pooled Sperm Dilution Worksheet
- A blank Excel sperm dilution worksheet for use with Nanodrop 2000 cell counts as described in Diluting Pooled Sperm based on NanoDrop Cell Counts